Composite
sigIL-bFGF

Part:BBa_K4211004:Design

Designed by: Peisong Wu   Group: iGEM22_BNUZH-China   (2022-08-19)


bFGF secretion system


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

  • No BioBrick restriction enzyme cut sites in coding region.
  • Constructed by PCR.

Forward primer

5'- CTC GAC GGT ACC GCG GGC CCG GGA TCC GCC ACC ATG TAC AG -3'

Reverse primer

5'- GCT GAT TAT GAT CTA GAG TCG CGG CCG CTT AAT GGT G -3'


Source

The BNUZH-China iGEM team 2022 designed and built.

References

Powers, C. J., McLeskey, S. W. & Wellstein, A. Fibroblast growth factors, their receptors and signaling. Endocr Relat Cancer 7, 165-197 (2000). https://doi.org:10.1677/erc.0.0070165